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Bioconjugate Techniques, Second Edition, by Greg T. Hermanson
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Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.
*A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
*Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
*More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates
*Includes entirely new chapters on the latest areas in the field of bioconjugation as follows:
Microparticles and nanoparticles
Silane coupling agents
Dendrimers and dendrons
Chemoselective ligation
Quantum dots
Lanthanide chelates
Cyanine dyes
Discrete PEG compounds
Buckyballs,fullerenes, and carbon nanotubes
Mass tags and isotope tags
Bioconjugation in the study of protein interactions
- Sales Rank: #1144039 in Books
- Published on: 2008-05-02
- Original language: English
- Number of items: 1
- Dimensions: 1.74" h x 7.51" w x 9.22" l, 4.03 pounds
- Binding: Paperback
- 1323 pages
Review
"This is a well-organized and well-written book; it is readable and comprehensible to the novice, but its content and stylistic approach seem sufficiently sophisticated to appeal to active, knowledgeable workers in the field. It is an impressive compilation of useful theoretical and practical information that is not readily available elsewhere in a single volume."
--Joseph G. Cannon in JOURNAL OF MEDICINAL CHEMISTRY
"The presentation throughout is that of a laboratory manual. Well-illustrated descriptions of a particular chemical reaction are followed by a detailed protocol and list of references extending into 1994. The coverage of reagents and methods is comprehensive, and sources are given for commercially available compounds."
--Nature
Most helpful customer reviews
2 of 2 people found the following review helpful.
A must-have, comprehensive reference book that will empower you to build anything biologically relevant!
By Shann Yu
This book is not one that's meant to be read from cover to cover. For the academic seeking to build a molecule or put together particulate systems, conjugate proteins onto some sort of substrate, modify surfaces to produce certain biologically active moieties, etc., this book is a must-have reference that contains:
- Clear explanation of what kind of chemistry is involved
- Concise, easy to follow outline of reaction conditions that must be kept in mind.
- Good figures and diagrams of the reactions of interest
- Well-researched protocols that serve as a point of reference for your experimental plans.
This edition is a significant upgrade from the previous edition, with newly written chapters on microspheres and quantum dots, among some. Whenever I need help figuring out how to attach certain biomolecules to various surfaces in my research, this is my go-to book, and I have seen plenty of labs in my area that have at least one copy of this book on the shelf.
Overall, probably too heavy to read from cover to cover, but makes for an awesome desk reference for grad students and researchers in biologicalk and biotech-related disciplines.
2 of 2 people found the following review helpful.
Simply The Best!
By Yi Dong
This is the best book on this topic. I use it as a reference often as I can't mesmerize reaction/reagent in my head. This book has all the major topics covered in sufficient details. Once you have this book, you can't live without it.
2 of 2 people found the following review helpful.
You need this book to modify proteins
By P. Luu
It's an excellent reference to those practice conjugation of proteins and nucleic acids. It provides detail experiment conditions and tips to set up the experiment correctly and successfully.
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